human str cell line validation kit Search Results


human hipsc cell line 18  (TaKaRa)
93
TaKaRa human hipsc cell line 18
Human Hipsc Cell Line 18, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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neurotrophic factor gdnf elisa kit  (Boster Bio)
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Boster Bio neurotrophic factor gdnf elisa kit
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Neurotrophic Factor Gdnf Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csb e04565h  (Cusabio)
93
Cusabio csb e04565h
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Csb E04565h, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio y00275  (TaKaRa)
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TaKaRa bio y00275
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Bio Y00275, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human fetal nscs  (TaKaRa)
92
TaKaRa human fetal nscs
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Human Fetal Nscs, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human ipsc line 12  (TaKaRa)
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TaKaRa human ipsc line 12
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Human Ipsc Line 12, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human neural hindbrain stem cells  (TaKaRa)
92
TaKaRa human neural hindbrain stem cells
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Human Neural Hindbrain Stem Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lines hipsc22 takara bio  (TaKaRa)
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TaKaRa cell lines hipsc22 takara bio
A. Light field of SSCs cultured with melatonin and <t>GDNF,</t> bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.
Cell Lines Hipsc22 Takara Bio, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gdnf picokine elisa kit boster biological technology  (Boster Bio)
93
Boster Bio gdnf picokine elisa kit boster biological technology
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Gdnf Picokine Elisa Kit Boster Biological Technology, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hesc line sa121  (TaKaRa)
93
TaKaRa hesc line sa121
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Hesc Line Sa121, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cell line nucleofector kit c vaca-1004  (Amaxa)
90
Amaxa human cell line nucleofector kit c vaca-1004
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Human Cell Line Nucleofector Kit C Vaca 1004, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human gdnf (glial cell line derived neurotrophic factor) elisa kit  (Elabscience Biotechnology)
90
Elabscience Biotechnology human gdnf (glial cell line derived neurotrophic factor) elisa kit
Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using <t>ELISA,</t> indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor <t>(GDNF)</t> in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.
Human Gdnf (Glial Cell Line Derived Neurotrophic Factor) Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Cell Culture, Quantitative RT-PCR, Western Blot

A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics

Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using ELISA, indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor (GDNF) in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.

Journal: Journal of Neural Engineering

Article Title: Enhancing facial nerve regeneration with scaffold-free conduits engineered using dental pulp stem cells and their endogenous, aligned extracellular matrix

doi: 10.1088/1741-2552/ad749d

Figure Lengend Snippet: Formation and characterization of aligned DPSC conduits. (A) Images of conduit formation. (B) Hematoxylin and eosin (H&E) staining of longitudinal dental pulp stem cell (DPSC) conduit sections showed that both unaligned and aligned conduits were solid and cellular, with those formed from the aligned DPSC sheets maintaining their nuclear alignment. (C) Picrosirius staining (PSR), imaged with brightfield (BF) and polarized light microscopy, indicated that the conduits from the aligned DPSC sheets also preserved their extracellular matrix (ECM) alignment. (D) Scanning electron microscopy (SEM) further confirmed that the aligned DPSC conduits contained an oriented matrix which was absent from the unaligned conduits. (E) Neurotrophic factor (NTF) expression, quantified using ELISA, indicated similar levels of brain-derived neurotrophic factor) and glial cell line derived neurotrophic factor (GDNF) in the conduits. Paired t-test indicated that the NTF expression levels were statistically similar between the unaligned and aligned conduits. (F) Immunofluorescence staining against BDNF and GDNF demonstrated that this NTF expression was present throughout the matrix of the conduits. (G) Tensile tests produced load-displacement curves such as those represented here. From these curves the load at failure (N), stiffness (N mm −1 ), and % displacement were calculated, overall showing that these factors for the conduits were on the same order of magnitude as rat facial nerves. Statistical comparisons for the mechanical assessments were performed using one-way analysis of variance (ANOVA) and Tukey’s post-hoc tests (*: p -value < 0.05, **: p -value < 0.01). Scale bars: (B) low magnification = 500 μ m, high magnification = 200 μ m, (C) BF = 150 μ m, polarized = 150 μ m, (D) ×2000 = 15 μ m, ×10 000 = 2 μ m, (F) 100 μ m.

Article Snippet: Then human BDNF (PicoKine ELISA Kit, Boster Biological Technology) and GDNF (PicoKine ELISA Kit, Boster Biological Technology) ELISA kits were used to measure the NTF concentrations in the lysates.

Techniques: Staining, Light Microscopy, Electron Microscopy, Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay, Immunofluorescence, Produced