Journal: Journal of advanced research

Article Title: A cocktail hydrogel promoting the functional interneurons regeneration of human neural progenitor cells for brain injury therapy.

doi: 10.1016/j.jare.2025.05.063

Figure Lengend Snippet: Fig. 2. The in vitro release behavior of neural inducers and biocompatibility of BGA@GelMA hydrogel. (A to C) The relative release profile of BDNF, GDNF, and cAMP of the BGA@GelMA hydrogel measured with ELISA kit, n = 3. (D) Schematic representation of 3D culture system via the BGA@GelMA and the Matrigel hydrogel. (E) Flow cytometry was used to detect APC of cell apoptosis. (F) Statistical diagram of apoptotic cell distribution, n = 3, ***p = 0.0005. (G) Quantitative analysis of live cells and dead cells per field in the live/dead assay, n = 3. (H) Representative live/dead images of hNPCs co-cultured with the BGA@GelMA hydrogels and the Matrigel hydrogels after 14 days.

Article Snippet: The release kinetics of neural inducers in BGA@GelMA hydrogel were assessed using the human BDNF valukine enzyme-linked immunosorbent assay (ELISA) kit (VAL136, R&D Systems), GDNF ELISA kit (CSB-E04565h, Cusabio) and cAMP ELISA kit (CSBE04488h, Cusabio), respectively.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Live Dead Assay, Cell Culture

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Cell Culture, Quantitative RT-PCR, Western Blot

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics

Journal: ACS Biomaterials Science & Engineering

Article Title: Promoting and Orienting Axon Extension Using Scaffold-Free Dental Pulp Stem Cell Sheets

doi: 10.1021/acsbiomaterials.1c01517

Figure Lengend Snippet: NTF expression by DPC sheets. (A) Analysis of the NTF mRNA expression using qPCR. Biological variability was evaluated by isolating mRNA from cell sheets formed using different human donor cells, denoting different human samples with HS. (B) Total BDNF, GDNF, and NT3 protein produced by DPC sheets, quantified by performing ELISA on the conditioned media of the sheets. Total protein was also normalized to average number of cells per cell sheet. The data represent an average of sheets formed using four different donor cells, and error bars indicate the standard deviation across biological replicates (*: p -value < 0.05, **: p -value < 0.005).

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for human BDNF (PicoKine ELISA Kit, Boster Biological Technology), GDNF (PicoKine ELISA Kit, Boster Biological Technology), and NT-3 (RayBiotech) were used to measure the protein concentration of the DPC sheet CM.

Techniques: Expressing, Produced, Enzyme-linked Immunosorbent Assay, Standard Deviation